Read a chamber image or compare early and final images from a canine CRP latex-agglutination assay.
Use a stationary disc, fixed phone holder and diffuse transmitted light. Include the same reference window in every image. Keep timing, camera settings and geometry identical for standards and samples.
Photo uploads have unknown camera settings. Browser camera controls vary by device; native iPhone capture offers stronger control.
Orange S = chamber; blue R = reference. Each box is 8% of the image dimensions. Avoid bubbles, beads settled on the floor, glare and chamber edges. Selections apply to both images.
Include a zero-CRP blank and at least two nonzero standards. Match the selected measurement mode and color channel. Signals must increase with concentration; no extrapolation is used.
Add an image and choose chamber and reference regions.
This is an image attenuation index, not physical absorbance or a direct measurement at 600 nm. Neither a zero standard nor interpolation establishes a saliva detection limit. Visible cloudiness alone does not confirm CRP.
Records retain their original result, calibration, image regions and capture details. They are not changed when you edit the current calibration.
Canine CRP + anti-canine CRP antibodies on latex beads → aggregates → increased turbidity. The beads carry antibodies, not CRP. Small aggregates can change light transmission without forming visible clumps.
Acuvet kit AC/CCRPT02 includes reagents, calibrator and controls. The published serum method uses 3 µL sample, 230 µL buffer and 70 µL immunoparticles, measured at 600 nm. The study reported a quantification limit of 1.4 mg/L.
Manufacturer · Published methodReagent kit 1501 contains assay buffer and antibody-coated polystyrene particles. Calibrator 1551 and controls 1519 are separate. Established for serum/plasma; measurement settings and sensitivity depend on the analyzer.
Manufacturer and assay detailsSaliva remains a development step. Verify reagent blanks, known canine CRP standards and matched unspiked/spiked saliva with a laboratory optical reader before relying on photographs. A strong spike demonstrates the reaction, not sensitivity to natural salivary CRP. Canine saliva study
Disc concept: sample chamber → channel → transparent reaction chamber with suspended antibody-coated beads and buffer. Dry-bead storage, mixing and rehydration require separate development.